团队负责人 团队负责人

最小化 最大化

 

李福利

研究员 

分子微生物工程

研究组负责人

        1998年毕业于兰州大学,2003年在山东大学微生物技术国家重点实验室获得微生物学专业博士学位。2005年9月至2007年9月在德国马普微生物研究所从事博士后研究,获克虏伯"中国青年学者"奖学金和马普奖学金,2009年10月至12月在慕尼黑工业大学微生物研究所开展合作研究。作为负责人或主要参与者,承担国家863计划,国家自然科学基金重大项目及面上项目,省部级10余项科研项目。山东省微生物学会理事,中国微生物学会国际工作委员会委员,中科院青岛生物能源所学位委员会委员。自2015年起任Frontiers in Biotechnology and Bioengineering 编委;发表论文60余篇,SCI论文40余篇;主编英文专著一部,申请专利11件,目前获得授权6件。山东大学和山东省优秀博士论文获得者,2015年获得山东省自然科学杰出青年基金。

招聘信息 招聘信息

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招聘博后2-3名

岗位任务团队主要从事微生物生物化学与分子生物学研究,尤其是微生物能量代谢、多糖水解、油脂合成机制等,因工作需要,现招收具有酶催化机制、蛋白质晶体结构研究,或代谢工程、合成生物学、发酵工程研究,或基因组、转录组分析等研究经历的博后2-3名,除享受研究所正常工资待遇,还有青岛市30-40万补贴。

专业背景要求:生化与分子生物学、酶学、组学、发酵工程、代谢工程等。

年龄要求:低于35岁

联系方式 联系方式

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地址:山东省青岛市松岭路189号

邮编:266101

电话:0532-80662655/80662656

邮箱:lifl@qibebt.ac.cn

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New Wirewalking Mode of Cellulose Degradation Proposed

Cellulose, the earth's most abundant biopolymer, is responsible for the structural integrity of plants. Processive cellulases are essential in crystalline cellulose degradation as they continuously catalyze and slide along the cellulose chain before dissociating.

There are two types of processive cellulases: exoglucanases and processive endoglucanases. The working mode of exoglucanases is named as threading mode. Whereas, the working mode of processive endoglucanases has not been proposed due to their open cleft in the catalytic module.

Recently, a research team led by Prof. LI Fuli from Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT) of the Chinese Academy of Sciences proposed a wirewalking mode for processive degradation of crystalline cellulose by an endoglucanase (Fig. 1), which provided insights for rational design of industrial cellulases.

CcCel9A, a processive endoglucanase from the bacterium Clostridium cellulosi, comprises a glycoside hydrolase (GH) family 9 module with an open cleft and five carbohydrate-binding modules (CBMs).

The results demonstrate that the C-terminal CBM3b and three CBMX2 modules enhance the productive adsorption to substrate, while the CBM3c adjacent to the GH9 is tightly bound to 11 glucosyl units, thereby extending the catalytic cleft to 17 subsites, which facilitates decrystallization by forming a supramodular binding surface.

In the open cleft, the strong interaction force between substrate-binding subsites and glucosyl rings enables cleavage of the hydrogen bonds and extraction of the single cellulose chain. In addition, subsite −4 is capable of drawing the chain to its favored location. Cellotetraose is released from the open cleft as the initial product to achieve high processivity, which is further hydrolyzed to cellotriose, cellobiose and glucose by the catalytic cleft of the endoglucanase.

"CcCel9A recruits multiple modules, whereby the GH9 and CBM3c together form an open cleft and four product-releasing glucosyl sites, consisting of up to 17 glucosyl binding subsites, to achieve processivity," said Prof. LI.

The study entitled "Processive Degradation of Crystalline Cellulose by a Multimodular Endoglucanase via a Wirewalking Mode" was published in Biomacromolecules.

The research was supported by the National Natural Science Foundation of China and the Shandong Province Natural Science Funds for Distinguished Young Scholars.

 

 

Fig. 1. Working model of CcCel9A. (Image by ZHANG Kundi)

团队简介 团队简介

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分子微生物工程研究组致力于 研究微生物与微藻的生物化学,生理学和代谢工程改造,尤其是嗜热微生物的糖苷水解酶,酵母代谢工程,微藻油脂积累机制为生物能源的开发提供上游工艺和技术支持。

     

团队现有工作人员9名,其中研究员2人,副研究员4人,助理研究员2人;其中博士以上6人,硕士2人。另有博士后3人,博士研究生3人,硕士研究生6人。团队成员学科背景分布于微生物学、生物化学、分子生物学、遗传学、细胞生物学等。

 

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